Call Us Today
Special Corporate Bulk 
Pricing Available

1-800-440-6695



    Contact Us

Department Of Transportation (DOT) Drug Testing

Transportation Employee Testing Act of 1991 requires drug and alcohol testing of safety-sensitive transportation employees in aviation, trucking, railroads, mass transit, pipelines, and other transportation industries. DOT publishes rules on who must conduct drug and alcohol tests, how to conduct those tests, and what procedures to use when testing. These regulations cover all transportation employers, safety-sensitive transportation employees, and service agents – roughly 10 million people. Encompassed in 49 Code of Federal Regulations (CFR) Part 40, the Office of Drug & Alcohol Policy & Compliance (ODAPC) publishes, implements, and provides authoritative interpretations of these rules. 
Laboratory DOT Drug Test
$
69.99    
As an employee or employer, how do I know if I am subject to DOT testing?

Generally, DOT regulations cover safety-sensitive transportation employers and employees.  Each DOT agency (e.g. FRA, FMCSA, FTA, FAA, and PHMSA) and the USCG have specific drug and alcohol testing regulations that outline who is subject to their testing regulations.
Standard DOT 5 Panel Drug Test:
Drug Group

Amphetamines
(amphetamine and 
methamphetamine)

Cocaine metabolite

Marijuana metabolites

Opiates (codeine and
morphine)

Phencyclidine
Screen Cutoff Level


300 pg/mg


300 pg/mg

1 pg/mg

500 pg/mg


300 pg/mg
GC/MS Confirmation


300 pg/mg


300 pg/mg

0.1 pg/mg

500 pg/mg


300 pg/mg

Department Of Transportation Reference Guides

DOT Order 3910.1D - Drug and Alcohol-Free Departmental Workplace Program
DOT Drug and Alcohol Testing Guide (For DOT Employees)